Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003830_z5s1 ACGGAGAACAGAATTGCCCTGCACTGCATCAG(+ACTCAC)GATCCCTCTGTAAGCCCTGCCATGGGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605003829_438c CACTATCCAGGAGAGCCAGTATGGTTTGTTGG(+ACTCAC)GTACCATCTGTAGCAAATAACATCCAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605003827_efn6 GCTGTACAGCTGGATGTTATTTGCTACAGATG(+ACTCAC)GTACCCAACAAACCATACTGGCTCTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605003825_387r CAATCTGGCTAAGTCAGCTCCTAGGGCCTTGG(+ACTCAC)GATCCTCAGGAACTTGGGCATCATTGGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605003823_yt6v CTAACTTGGCCAATGATGCCCAAGTTCCTGAG(+ACTCAC)GATCCCAAGGCCCTAGGAGCTGACTTAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605003822_j5x5 AAAGGTTTCAAGGATTTGAGTTCCCTTCACTG(+ACTCAC)GATCCCAGAGATGATCATAGTGGCCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240605003820_zlrz CTGCCATCATGGGCCACTATGATCATCTCTGG(+ACTCAC)GATCCAGTGAAGGGAACTCAAATCCTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605003819_km10 ATAAAGGATGCTCAGGAACTGGCATGTAGCTG(+ACTCAC)GTACCACCGAGAACTCAATGGGCAGAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605003817_s7b3 TCTTGGATACTCTGCCCATTGAGTTCTCGGTG(+ACTCAC)GTACCAGCTACATGCCAGTTCCTGAGCATCC NGG 10 8 17 8 24 0 100 5 10
    240605003816_8231 ATTTTATCTTGTCCTTGATAATCTGGCTTAAG(+ACTCAC)GATCCCTTTAGTATTTACAGAGTACTGTTGT NGG 10 8 17 8 24 0 100 5 10