Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003726_0pas ATAAATAAATAAATAAGAGATATTTATGCCAG(+ACTCAC)GTACCCAGTCACAGCTGTAGAACGCTGATTA NGG 10 8 17 8 24 0 100 5 10
    240605003725_mva6 CCAGACCCTGAACCTCCACATGCAGCAGTGTG(+ACTCAC)GTACCATGTCAGAGCGCCAGAGCAGAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605003723_uu3k CAGATAGCACTCTGCTCTGGCGCTCTGACATG(+ACTCAC)GTACCACACTGCTGCATGTGGAGGTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605003722_qg3d CATGAAAGAGTTATTAGCCACCAAGTATGCTG(+ACTCAC)GTACCTATAATCCCAGAACACAGAAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605003721_cf49 CTGCCTCAGCCTTCTGTGTTCTGGGATTATAG(+ACTCAC)GTACCAGCATACTTGGTGGCTAATAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240605003719_8qtf GCATGAAGTTATCTGTGAGATGAGGGTAGGAG(+ACTCAC)GATCCTGGGAGTCAGGACCACCCAGAACCAA NGG 10 8 17 8 24 0 100 5 10
    240605003717_ej3y GTGGGTTGGTTCTGGGTGGTCCTGACTCCCAG(+ACTCAC)GATCCTCCTACCCTCATCTCACAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240605003713_nxeu CCTGGAGGGCCTGGGCTGGAAATTTGCCCCTG(+ACTCAC)GATCCAGAACCCCATTCGTTTTACAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605003712_h5od CCTATCCCAACTGTAAAACGAATGGGGTTCTG(+ACTCAC)GATCCAGGGGCAAATTTCCAGCCCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605003711_qovl TGCGTTCTTGCCATGAGCGAGCTGACTATCAG(+ACTCAC)GTACCTGTTCTCGCTCTGTCTGGGAACTGTG NGG 10 8 17 8 24 0 100 5 10