Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003619_ay3t CCCAGTAGGTACCGGCCAACAGAGTATCACAG(+ACTCAC)GATCCAGAGGTCCAACAGCCAAGGAGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605003618_4v2y GTATGTAAGCTGGACAAGATTCTACAGTAGAG(+ACTCAC)GTACCTACATGCCTGCCAGGGGAACCGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605003617_acen AGATGACAACGGTTCCCCTGGCAGGCATGTAG(+ACTCAC)GTACCTCTACTGTAGAATCTTGTCCAGCTTA NGG 10 8 17 8 24 0 100 5 10
    240605003615_1vhi TCAGACGTTGTTTTGACCAAAGTGTTCACTGG(+ACTCAC)GATCCTCCAGGAAGTACCAGGGAACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605003613_zsvw GTTATGCTGCAGTTCCCTGGTACTTCCTGGAG(+ACTCAC)GATCCCAGTGAACACTTTGGTCAAAACAACG NGG 10 8 17 8 24 0 100 5 10
    240605003612_0mer CACATTAGTTAGTAACCACAGAACTGGTTTTG(+ACTCAC)GATCCTGGAATTAAAGCATTAATATCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605003610_ygn1 ATTTACCTTTGATATTAATGCTTTAATTCCAG(+ACTCAC)GATCCAAAACCAGTTCTGTGGTTACTAACTA NGG 10 8 17 8 24 0 100 5 10
    240605003609_9uz6 GTGTCAGTCTCCAAGCTTACCTTTTATACAAG(+ACTCAC)GATCCCAGTCTTGGGTTGGGGCTCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605003607_rv0y AGTAGACAGGGTGAGCCCCAACCCAAGACTGG(+ACTCAC)GATCCTTGTATAAAAGGTAAGCTTGGAGACT NGG 10 8 17 8 24 0 100 5 10
    240605003605_yt9c GCTATTTCTTGCTCCAGGAGCATGGCTACCAG(+ACTCAC)GATCCCCAATATTCAGCATCAACTGTTTTCA NGG 10 8 17 8 24 0 100 5 10