Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003636_y3fl TTGTAACTCCAGTTCTATGATGTTGTCCATGG(+ACTCAC)GTACCAGGTACACATGTGATGCAGACATATG NGG 10 8 17 8 24 0 100 5 10
    240605003634_4fl1 CTGCACATATGTCTGCATCACATGTGTACCTG(+ACTCAC)GTACCCATGGACAACATCATAGAACTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605003631_4jca TTGTAACTCCAGTTCTATGATGTTGTCCATGG(+ACTCAC)GTACCAGGTACACATGTGATGCAGACATATG NGG 10 8 17 8 24 0 100 5 10
    240605003630_fucg ATTGTGCATTTGCCATAGGGTCCTTGTTAAAG(+ACTCAC)GTACCATAAAAATAATAATTTTGTAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240605003629_2v73 GAGTTTTTTATTACAAAATTATTATTTTTATG(+ACTCAC)GTACCTTTAACAAGGACCCTATGGCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240605003627_x2y3 TCTGCCTGCCTGCAGGTTGGCCATGCTCCTTG(+ACTCAC)GATCCTGTAGGTTAACATTCATGCTTTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605003626_91x8 CTTCATCAGAAAGCATGAATGTTAACCTACAG(+ACTCAC)GATCCAAGGAGCATGGCCAACCTGCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605003624_tii0 GACCTCTGGATCCTGTGATACTCTGTTGGCCG(+ACTCAC)GTACCTACTGGGCTCCCTCAGCCCTATACCT NGG 10 8 17 8 24 0 100 5 10
    240605003623_6i1e CCCACAGGTATAGGGCTGAGGGAGCCCAGTAG(+ACTCAC)GTACCGGCCAACAGAGTATCACAGGATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605003621_72io TGATGTGGGCCTCCTTGGCTGTTGGACCTCTG(+ACTCAC)GATCCTGTGATACTCTGTTGGCCGGTACCTA NGG 10 8 17 8 24 0 100 5 10