Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003740_yw5a CCCATGCACACAGTGGCCTCAGTAGCTGGTAG(+ACTCAC)GATCCTGAGTATTTGGGTAATACGTGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605003739_k5sa CAGCACACTGAGAACATCTTAGTGAGTACACG(+ACTCAC)GTACCTTAACTTGATATTTTTCAGCAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605003737_t8bh ACTTTACATTTGCTGAAAAATATCAAGTTAAG(+ACTCAC)GTACCGTGTACTCACTAAGATGTTCTCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605003736_2bei TTTCTCTTTTGAAGAGTATTCTTTACCTGCTG(+ACTCAC)GATCCAAGAAATTCACATTGATTTCTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605003735_yf4a CTTCAATGGCAGAAATCAATGTGAATTTCTTG(+ACTCAC)GATCCAGCAGGTAAAGAATACTCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605003733_8jlc CAGCTCACAACCTTTATAAATCCATTTCAGGG(+ACTCAC)GATCCAACGCCTCCTTGCGTGTTGCACACAT NGG 10 8 17 8 24 0 100 5 10
    240605003732_f2xx AGCACATGTGTGCAACACGCAAGGAGGCGTTG(+ACTCAC)GATCCCCTGAAATGGATTTATAAAGGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605003730_yqqv TGTGACCAAATACATGGTCAGTTTTGGAGAAG(+ACTCAC)GTACCGTGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605003729_xayf CAAAGAATATACCTTCTTCTCAGCACCTCACG(+ACTCAC)GTACCTTCTCCAAAACTGACCATGTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240605003728_p60d ACAGTTAATCAGCGTTCTACAGCTGTGACTGG(+ACTCAC)GTACCTGGCATAAATATCTCTTATTTATTTA NGG 10 8 17 8 24 0 100 5 10