Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003756_zk2l CAAAGGCAGCCCACCTCCAGCTTTAATGGGGG(+ACTCAC)GATCCAGGGAGGGGAGGAGAGCAAGGCGGGA NGG 10 8 17 8 24 0 100 5 10
    240605003754_s2x1 GTCTGCAGCTCCATTACTTTTCTCAGTCAGTG(+ACTCAC)GATCCAAGGGACCAAGATCCATTGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240605003752_it2w ATTTGCATCTGCCAATGGATCTTGGTCCCTTG(+ACTCAC)GATCCACTGACTGAGAAAAGTAATGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605003750_q0mj AAAAAGCAGCGGCAGACAGGATTTTTGTCCAG(+ACTCAC)GATCCACTATCCAGCTTTAAGATGAGTCATT NGG 10 8 17 8 24 0 100 5 10
    240605003749_trjf GGTCGAATGACTCATCTTAAAGCTGGATAGTG(+ACTCAC)GATCCTGGACAAAAATCCTGTCTGCCGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605003747_1dj3 ATCTCTTTCGTGCAAACCACATTTCTACACTG(+ACTCAC)GTACCTCCTAAGTCCAGAAGTTGGCCTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605003746_so2o TGGGCACGGAGGCCAACTTCTGGACTTAGGAG(+ACTCAC)GTACCAGTGTAGAAATGTGGTTTGCACGAAA NGG 10 8 17 8 24 0 100 5 10
    240605003744_sagb GGTGTCCAGAGTGCCTGCAGACAGTGTGCCCG(+ACTCAC)GTACCTCTGTCTACCTTTTGTAACGTTACCG NGG 10 8 17 8 24 0 100 5 10
    240605003743_ahkr CCAGGCGGTAACGTTACAAAAGGTAGACAGAG(+ACTCAC)GTACCGGGCACACTGTCTGCAGGCACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605003741_2ljp TACCACCCACCACGTATTACCCAAATACTCAG(+ACTCAC)GATCCTACCAGCTACTGAGGCCACTGTGTGC NGG 10 8 17 8 24 0 100 5 10