Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003709_zcc8 TGTGACACAGTTCCCAGACAGAGCGAGAACAG(+ACTCAC)GTACCTGATAGTCAGCTCGCTCATGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605003707_syhu GGCTAAGGATGGCCCTAAACATCCATCCTTCG(+ACTCAC)GATCCTCCTGCCTCCTTTTCTCTAGTGCGGG NGG 10 8 17 8 24 0 100 5 10
    240605003706_p0rm TGATCCCCGCACTAGAGAAAAGGAGGCAGGAG(+ACTCAC)GATCCGAAGGATGGATGTTTAGGGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605003705_uq4c ACACCAACACATGAGCACAAGAGGCGGGTCAG(+ACTCAC)GTACCCACACTCCACACTGTTTGGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605003703_u7ci CCACATGAGAAGCCAAACAGTGTGGAGTGTGG(+ACTCAC)GTACCTGACCCGCCTCTTGTGCTCATGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605003702_o2gs TCTCCTAGCACAACAGTGCCAACAAGCTTCGG(+ACTCAC)GATCCCAGCCCAGGTCTGGGGAACTTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605003700_ve0m ACAGATGGCAAAGTTCCCCAGACCTGGGCTGG(+ACTCAC)GATCCCGAAGCTTGTTGGCACTGTTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605003658_llsw ACAAGGTAGCCCTCCACCAGGTCTTAGCGGCG(+ACTCAC)GATCCCGTGCACTGCAATCCCCACCCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605003657_rv0c ACACCTTCCTGGGTGGGGATTGCAGTGCACGG(+ACTCAC)GATCCGCCGCTAAGACCTGGTGGAGGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605003655_dbxd GGGGAAGTCAGAGGTAATGCTCTGGATAAGAG(+ACTCAC)GATCCAGTGGGCAGCTCCTGCCACGCACAGT NGG 10 8 17 8 24 0 100 5 10