Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002031_bomp GTAAACAGAGAAGAGAATAACATTCGAATATG(+ACTCAC)GTACCAACGGGACTTGTACAGGAGTGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605002030_9bvf ACTTATTAACAAGCCGAGCAGAATTTTTATAG(+ACTCAC)GATCCATCCATCCAGATTTATTACAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605002028_zvjb AAATGGTGTTCTGTAATAAATCTGGATGGATG(+ACTCAC)GATCCTATAAAAATTCTGCTCGGCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605002026_ilk2 TCTTGAGTTGATGATAACTGGAAGTCCACCTG(+ACTCAC)GTACCTGCCTTCCTGTTCATTTGTCTTCATT NGG 10 8 17 8 24 0 100 5 10
    240605002025_fx5y CTTTCAATGAAGACAAATGAACAGGAAGGCAG(+ACTCAC)GTACCAGGTGGACTTCCAGTTATCATCAACT NGG 10 8 17 8 24 0 100 5 10
    240605002023_s28r ATAGTAAAGTCTAAAAATGAGCAAGCCATTGG(+ACTCAC)GTACCAACAGGTTCAAATGTTAGCTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605002022_lpkc ACACCACTGCAAGCTAACATTTGAACCTGTTG(+ACTCAC)GTACCCAATGGCTTGCTCATTTTTAGACTTT NGG 10 8 17 8 24 0 100 5 10
    240605002020_nk5d GCTTCCCTGCCAGCCCGCATAGGAGGTGTGTG(+ACTCAC)GATCCTAATAGGTTGAAGTTTTTAAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605002019_pngq TCCTCCATGCTTTAAAAACTTCAACCTATTAG(+ACTCAC)GATCCACACACCTCCTATGCGGGCTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605002017_0cfb AGTCTCCTGAGTACTGGGTTTGCAGGTTTCGG(+ACTCAC)GTACCACATCAGCTTGATTTCATAATGGATA NGG 10 8 17 8 24 0 100 5 10