Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002116_52is AACATATAAGAGCTTTTGACAACTGATAACTG(+ACTCAC)GATCCTTCCAAATAAATGCATTTTGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240605002115_ya05 TGTCTTATTTGCCAGCTGAGAAAATGTACATG(+ACTCAC)GTACCATCATTAAGGTGGTCGGTATATAGGA NGG 10 8 17 8 24 0 100 5 10
    240605002114_8lsy TTTTTTCCTATATACCGACCACCTTAATGATG(+ACTCAC)GTACCATGTACATTTTCTCAGCTGGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240605002112_2lwt CTCTGCTTTCTCGGGGTTGCCACAGCAGACTG(+ACTCAC)GTACCTCATTTCACCACACCACTTAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605002111_dan3 ACACCTGTTCTTAAGTGGTGTGGTGAAATGAG(+ACTCAC)GTACCAGTCTGCTGTGGCAACCCCGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605002109_ghd7 ATTGCTTTAACCCAAGTTAAAACCAAGCTTGG(+ACTCAC)GATCCCATGCTTGCTGAGATGATTGACGGCT NGG 10 8 17 8 24 0 100 5 10
    240605002108_17oe ATGACAGCCGTCAATCATCTCAGCAAGCATGG(+ACTCAC)GATCCCAAGCTTGGTTTTAACTTGGGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605002106_dcx6 CCAGTGGAAAGCACTATCTAGATCACACTTTG(+ACTCAC)GATCCCATTAGCCAAATGCACAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605002104_ax03 TAATTTGGACATTCTGTGCATTTGGCTAATGG(+ACTCAC)GATCCAAAGTGTGATCTAGATAGTGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605002102_ot99 TGACTCAATTACAAATCGATTCCAATTCCAGG(+ACTCAC)GTACCTCACAGCTCTATTGCTACCGCCAATT NGG 10 8 17 8 24 0 100 5 10