Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002206_odwg ATAATTTTGGAAAATATAATGCTATGGGAGAG(+ACTCAC)GTACCTGCTAACTCCAGGATGCCTGTTTATA NGG 10 8 17 8 24 0 100 5 10
    240605002205_n3zw CAGCCTGATACAACAGGCAGTGTCCTTCTTCG(+ACTCAC)GATCCCATCTGGGGATGCTGCTCGCCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605002203_6poy GAGGTGTCTGGGCGAGCAGCATCCCCAGATGG(+ACTCAC)GATCCGAAGAAGGACACTGCCTGTTGTATCA NGG 10 8 17 8 24 0 100 5 10
    240605002202_88mj TATCACATAGTCGCAGGTCATAAAGGAATGGG(+ACTCAC)GATCCAAAAGCAGTTTTCATCTTCAGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605002200_drjc CTCTAAGACTCTGAAGATGAAAACTGCTTTTG(+ACTCAC)GATCCCCATTCCTTTATGACCTGCGACTATG NGG 10 8 17 8 24 0 100 5 10
    240605002159_a65z TCTCTCTTGTAACTTAGAATACAATGAAAATG(+ACTCAC)GATCCAGCCATCATGGGGTTATTGGGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605002158_rjrj AAACAACTTTCCCAATAACCCCATGATGGCTG(+ACTCAC)GATCCATTTTCATTGTATTCTAAGTTACAAG NGG 10 8 17 8 24 0 100 5 10
    240605002156_qm3j CAGGGAGGCTTGGTAGTTTCTCTGTGTAATAG(+ACTCAC)GATCCAGTGAACTTCAGGTTTCTCCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605002155_6fk4 GGAGAAACAAAGGAGAAACCTGAAGTTCACTG(+ACTCAC)GATCCTATTACACAGAGAAACTACCAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605002152_i9v2 GAGACTCCTGCAAAGGCTATGAAACTCCCAGG(+ACTCAC)GATCCCAGAGGGCTGGAATCCTGAGATGTCC NGG 10 8 17 8 24 0 100 5 10