Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002235_04ov ATTTACAATACTCAGACCCGGCAGGAGTTAAG(+ACTCAC)GTACCAGATACCCTGTGAGCGGGTGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605002234_a8nu ACCTCATACAAAACTTGTTCAACAGTCTAGTG(+ACTCAC)GTACCAACAGCGTTGAGATGGCAGTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240605002232_m2pc TGTAGCGCAAAACTGCCATCTCAACGCTGTTG(+ACTCAC)GTACCACTAGACTGTTGAACAAGTTTTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605002230_90su TGATCATTATGACAGGTCGCTGCCCTCCAGTG(+ACTCAC)GTACCACTGACCTACAGAAGGACATCATGGC NGG 10 8 17 8 24 0 100 5 10
    240605002228_sz4g GCTCTGCCATGATGTCCTTCTGTAGGTCAGTG(+ACTCAC)GTACCACTGGAGGGCAGCGACCTGTCATAAT NGG 10 8 17 8 24 0 100 5 10
    240605002227_vmrx GCAGCTCAGGGTTGGGGCCTAGTTACAGTTTG(+ACTCAC)GATCCTTAGTCCAAGAATTTTGAGAACGTGA NGG 10 8 17 8 24 0 100 5 10
    240605002226_v90k TTTACTCACGTTCTCAAAATTCTTGGACTAAG(+ACTCAC)GATCCAAACTGTAACTAGGCCCCAACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605002224_djoc TGCAGGGTAAGATACCCATATACATTTTCCTG(+ACTCAC)GTACCATTTCCTCACTCAGAACAGTGACAGA NGG 10 8 17 8 24 0 100 5 10
    240605002223_gawq TCAAATCTGTCACTGTTCTGAGTGAGGAAATG(+ACTCAC)GTACCAGGAAAATGTATATGGGTATCTTACC NGG 10 8 17 8 24 0 100 5 10
    240605002221_fbh6 CCTGTTCATTAGTTTCTGACTCCTTGGGTAAG(+ACTCAC)GATCCATTAACATAAGCTCTAAAGTGAAATT NGG 10 8 17 8 24 0 100 5 10