Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002248_h5wk CAGGAGGAGCAGAGGGTTTGAGATGATGGTGG(+ACTCAC)GTACCATCAACCCACACTCACCTCTCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605002247_g5ua AGGCTTGCCAGAGAGGTGAGTGTGGGTTGATG(+ACTCAC)GTACCCACCATCATCTCAAACCCTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605002246_dmvv CTTTCTTTCTTTTTCTCCTTACTTATTTTGTG(+ACTCAC)GTACCTGAGACTAAACCCAGGGCCTCCCATG NGG 10 8 17 8 24 0 100 5 10
    240605002244_f9qz AGGCACATGGGAGGCCCTGGGTTTAGTCTCAG(+ACTCAC)GTACCACAAAATAAGTAAGGAGAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605002243_kc2d ACATCGGGAGAACTCTGAATGTTGTTCACAGG(+ACTCAC)GATCCTTAGGACACAGCTCCTCTGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605002241_22o0 AGTTTATAGAAACAGAGGAGCTGTGTCCTAAG(+ACTCAC)GATCCCTGTGAACAACATTCAGAGTTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605002239_3mr1 AGCTCAGTGATAGCACTTGCCTCGGGTGCACG(+ACTCAC)GTACCCTGGTTTAGATGCCCTGCACTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605002237_kzts ATTTTCTTTCAGTGCAGGGCATCTAAACCAGG(+ACTCAC)GTACCGTGCACCCGAGGCAAGTGCTATCACT NGG 10 8 17 8 24 0 100 5 10
    240605002236_t6g5 CTTAATCAGCTCACCCGCTCACAGGGTATCTG(+ACTCAC)GTACCTTAACTCCTGCCGGGTCTGAGTATTG NGG 10 8 17 8 24 0 100 5 10
    240605002235_04ov ATTTACAATACTCAGACCCGGCAGGAGTTAAG(+ACTCAC)GTACCAGATACCCTGTGAGCGGGTGAGCTGA NGG 10 8 17 8 24 0 100 5 10