Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002150_kbbw ATGCCGGACATCTCAGGATTCCAGCCCTCTGG(+ACTCAC)GATCCCTGGGAGTTTCATAGCCTTTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605002149_sr4x GGTCTTATATGATAATAACTACAGTAAGTGTG(+ACTCAC)GTACCCAGACTGAAAGATCAATACATGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605002148_2u7d GACATAGAGCATGTATTGATCTTTCAGTCTGG(+ACTCAC)GTACCACACTTACTGTAGTTATTATCATATA NGG 10 8 17 8 24 0 100 5 10
    240605002145_c5s5 TATTAGAGCAAGAGGCAGAGACTATGACCCAG(+ACTCAC)GTACCCCAGGGAAGATTCAGTCTAGAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605002142_ehoh CAAAATTACCTCTAGACTGAATCTTCCCTGGG(+ACTCAC)GTACCTGGGTCATAGTCTCTGCCTCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605002141_5isb GGCCCCAGAGGGAGGTGCAATCGACCTTTAGG(+ACTCAC)GATCCTCACCGACCAACTCAGCGAGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240605002140_2d2b TCCTCTTCTGTCTCGCTGAGTTGGTCGGTGAG(+ACTCAC)GATCCCTAAAGGTCGATTGCACCTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605002138_jejo AACCACAGCTCTGCTAGTCAGGACAACGTTAG(+ACTCAC)GTACCACTGACGACCCACAGGCCACTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605002137_i3lw ACTCGGCAGGAGTGGCCTGTGGGTCGTCAGTG(+ACTCAC)GTACCTAACGTTGTCCTGACTAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605002135_d3dj ATGTTGATTTAGGTATACACACTATAGAATGG(+ACTCAC)GATCCAAGATCTTTAACAGCTTGCTTGTATT NGG 10 8 17 8 24 0 100 5 10