Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002134_6u3k AAAAAAATACAAGCAAGCTGTTAAAGATCTTG(+ACTCAC)GATCCCATTCTATAGTGTGTATACCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605002131_laey GTAGGCGCGCTGGCCCGGCCACCGACACCGGG(+ACTCAC)GATCCCCGCTGCAGCGCCCAAACCGTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605002129_lv54 GCTAGAGGACACGGTTTGGGCGCTGCAGCGGG(+ACTCAC)GATCCCCGGTGTCGGTGGCCGGGCCAGCGCG NGG 10 8 17 8 24 0 100 5 10
    240605002127_k45o TTATCTTCACTTCCCCAGGAAGCAGTGACTGG(+ACTCAC)GATCCTTTGGGCATCGCCACTTTTGGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605002125_x8lq GTAACATCTTCCAAAAGTGGCGATGCCCAAAG(+ACTCAC)GATCCCAGTCACTGCTTCCTGGGGAAGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605002124_qqhy ATATTAAAAAACAGAAGTGGCAATTCAGTAAG(+ACTCAC)GATCCTTCCATTGTTCTTTATACAGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240605002122_neuq AGCATTTAAATCTGTATAAAGAACAATGGAAG(+ACTCAC)GATCCTTACTGAATTGCCACTTCTGTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605002121_7r97 TATTACATATTTCTTACGGAATAGTGGGTTAG(+ACTCAC)GTACCCAATTTCTCATCTCACTTCGTAATTT NGG 10 8 17 8 24 0 100 5 10
    240605002119_2vwz CATACAAATTACGAAGTGAGATGAGAAATTGG(+ACTCAC)GTACCTAACCCACTATTCCGTAAGAAATATG NGG 10 8 17 8 24 0 100 5 10
    240605002117_69q8 ACATTTGTTAACAAAATGCATTTATTTGGAAG(+ACTCAC)GATCCAGTTATCAGTTGTCAAAAGCTCTTAT NGG 10 8 17 8 24 0 100 5 10