Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605002220_75u3 TTTTTAATTTCACTTTAGAGCTTATGTTAATG(+ACTCAC)GATCCTTACCCAAGGAGTCAGAAACTAATGA NGG 10 8 17 8 24 0 100 5 10
    240605002218_fjqi AACAGCTCCTCTGAAAGGTTAATTAAGAGGTG(+ACTCAC)GTACCCAAATCAAGCCAAAATTTTATTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605002217_75oh TACATTTGAAATAAAATTTTGGCTTGATTTGG(+ACTCAC)GTACCACCTCTTAATTAACCTTTCAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605002215_1ddr TCCTCGGGGCGGAGGCTGCCCTTCTCAGGCAG(+ACTCAC)GATCCTTCTGATCCATGGAACTTCCTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605002214_wk8u TGTGGCACAAAGGAAGTTCCATGGATCAGAAG(+ACTCAC)GATCCTGCCTGAGAAGGGCAGCCTCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605002213_6jw1 AGCATTTCTGAAGGTGGGAGATTCCTAATCTG(+ACTCAC)GATCCAGTTGAACTACAAGTACTGGATTCGG NGG 10 8 17 8 24 0 100 5 10
    240605002211_2jr9 TCATGCCGAATCCAGTACTTGTAGTTCAACTG(+ACTCAC)GATCCAGATTAGGAATCTCCCACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605002210_vo6f CCCCTAATTATTATTATTTTTTACATGTGATG(+ACTCAC)GTACCATAACATTTCTGGGACTTCTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605002209_i92v TACATTGCTGTAGAAGTCCCAGAAATGTTATG(+ACTCAC)GTACCATCACATGTAAAAAATAATAATAATT NGG 10 8 17 8 24 0 100 5 10
    240605002208_8649 TAGTTTATAAACAGGCATCCTGGAGTTAGCAG(+ACTCAC)GTACCTCTCCCATAGCATTATATTTTCCAAA NGG 10 8 17 8 24 0 100 5 10