Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221637_o8im GGATTGCCAGCCTCCCTCAGCAGGGGCACTTG(+ACTCAC)GTACCCCCATAAGGGTGGCATGCCCCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240604221635_s9yi CAAGTGGACTGGGGCATGCCACCCTTATGGGG(+ACTCAC)GTACCAAGTGCCCCTGCTGAGGGAGGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240604221633_wd4k AAAAGAGGGGGGTGGGGGAGCTCCATGGGGTG(+ACTCAC)GATCCCAGGCTGGCAGCAGGAAGGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604221631_z2jg GGCCTTCCAGCACCTTCCTGCTGCCAGCCTGG(+ACTCAC)GATCCACCCCATGGAGCTCCCCCACCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240604221629_v7ik TAAAAAGAAACTTATGATTTATTAGAGTACAG(+ACTCAC)GATCCAAAACACATTTTTAGAAAAATATAAA NGG 10 8 17 8 24 0 100 5 10
    240604221628_4kol GGCACTTTATATTTTTCTAAAAATGTGTTTTG(+ACTCAC)GATCCTGTACTCTAATAAATCATAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604221626_0wos TCCCTTGGTCTTTACTCTCTACCACAGACCCG(+ACTCAC)GTACCAGGTCTTGCCCACTTAGCTTAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240604221624_hkqs CACGGATTCTTAAGCTAAGTGGGCAAGACCTG(+ACTCAC)GTACCGGGTCTGTGGTAGAGAGTAAAGACCA NGG 10 8 17 8 24 0 100 5 10
    240604221622_ddre TGAGGCAATTGGTTTGCCCTGCTGGGATATGG(+ACTCAC)GATCCAGGCTTCGTATGCATGCTAGGCGAAC NGG 10 8 17 8 24 0 100 5 10
    240604221620_bann GTAGAGTTCGCCTAGCATGCATACGAAGCCTG(+ACTCAC)GATCCCATATCCCAGCAGGGCAAACCAATTG NGG 10 8 17 8 24 0 100 5 10