Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221618_do5a GGGCAGCCACCCCATATCCCATGGGGCGGGAG(+ACTCAC)GATCCAGTTAGGAGCTGAAAACCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604221617_ef9d TCTTGTCCAGCAGGGTTTTCAGCTCCTAACTG(+ACTCAC)GATCCTCCCGCCCCATGGGATATGGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240604221616_upzh CTACAAACCTTGACTAATGGCACAGTGCACAG(+ACTCAC)GTACCAAGTGCCCCACCCCACCCCAGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240604221614_nhod TGATTGGGGACTGGGGTGGGGTGGGGCACTTG(+ACTCAC)GTACCTGTGCACTGTGCCATTAGTCAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240604221612_b1aj GGTTGAAATTCAAGATAAGAGCTCAGAGCTTG(+ACTCAC)GATCCAGACAGGAGCAGGGGCAGGTGGATGT NGG 10 8 17 8 24 0 100 5 10
    240604221611_07bn CACCAACATCCACCTGCCCCTGCTCCTGTCTG(+ACTCAC)GATCCAAGCTCTGAGCTCTTATCTTGAATTT NGG 10 8 17 8 24 0 100 5 10
    240604221609_it6g GACATTGTGCTCATCTCGATGTCAGCAAAAGG(+ACTCAC)GATCCACTCCTTCCTATGAGCAAACCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240604221608_1648 AAACAGACCAGGTTTGCTCATAGGAAGGAGTG(+ACTCAC)GATCCCTTTTGCTGACATCGAGATGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240604221606_llzy CTTTGCTGCTGCAAGGAGAATGTCTCTCTTGG(+ACTCAC)GTACCAGTGGGTCCTTCACACCTTCCCACAA NGG 10 8 17 8 24 0 100 5 10
    240604221604_xs5v TGGTGTTGTGGGAAGGTGTGAAGGACCCACTG(+ACTCAC)GTACCCAAGAGAGACATTCTCCTTGCAGCAG NGG 10 8 17 8 24 0 100 5 10