Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221546_kzp5 TAACTTGTTTCATTTCTTTTCAGATCCCTAAG(+ACTCAC)GATCCCCCCACCCCCCAATGCCGCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240604221544_lxjg TTCAGAGTTGGAGCGGCATTGGGGGGTGGGGG(+ACTCAC)GATCCTTAGGGATCTGAAAAGAAATGAAACA NGG 10 8 17 8 24 0 100 5 10
    240604221542_xaj0 TTCAGAGCCAGCACATCACCATCTGTTTCTGG(+ACTCAC)GTACCTACCTTATGCCTACCAGAGGGCTCGT NGG 10 8 17 8 24 0 100 5 10
    240604221541_lbgc ATAAAACGAGCCCTCTGGTAGGCATAAGGTAG(+ACTCAC)GTACCCAGAAACAGATGGTGATGTGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604221539_jrwx GGTGTCTGACCCTCCACCACCTCACCTTGTTG(+ACTCAC)GTACCTCCCTCCCTGAATTCCTGAGCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604221537_fdad AGAGTTGCTGGCTCAGGAATTCAGGGAGGGAG(+ACTCAC)GTACCAACAAGGTGAGGTGGTGGAGGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240604221535_urgu TGTCCCTCAGTATTGAACTTGGCTGCTTGTGG(+ACTCAC)GATCCAGTCCTGGAGGACAGGCATCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604221534_kk8v GCTAAGAACTTGATGCCTGTCCTCCAGGACTG(+ACTCAC)GATCCCACAAGCAGCCAAGTTCAATACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604221533_385a TTTGCTCTATTCCAGAGTCTGAGAGGTGTTTG(+ACTCAC)GTACCCGGTGTATCAGTAAATACTGCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240604221531_qxnj CACACGCTAGGCAGTATTTACTGATACACCGG(+ACTCAC)GTACCAAACACCTCTCAGACTCTGGAATAGA NGG 10 8 17 8 24 0 100 5 10