Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211405_vdhh TGCTCCAGAGTGGGAGGGAAAACAGTTGCGGG(+ACTCAC)GATCCAGGGAGCAGAACAACATTGGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240604211402_qp8z TTGCTGTCCTTGGTCTTACACAGAGAACCCAG(+ACTCAC)GTACCAGGCCACCTCCTTACTCAGAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240604211400_myh3 GCAGACTCTCTTCTGAGTAAGGAGGTGGCCTG(+ACTCAC)GTACCTGGGTTCTCTGTGTAAGACCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240604211357_bftb AGCTCACAGGCACCTCCAACTCAAGCTCCGGG(+ACTCAC)GATCCAACCTCCTTGCTGGTTTCTTTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604211356_77iw CCTGATGCCCAAAGAAACCAGCAAGGAGGTTG(+ACTCAC)GATCCCCGGAGCTTGAGTTGGAGGTGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604211355_pfyq GATCACAGATACTCAAAGAAGTGAGTGGTATG(+ACTCAC)GATCCACACATCCTCACAGTGTTATGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240604211353_1pr1 GGGAAATACACATAACACTGTGAGGATGTGTG(+ACTCAC)GATCCATACCACTCACTTCTTTGAGTATCTG NGG 10 8 17 8 24 0 100 5 10
    240604211352_9bjf CATTCACTTAAGGGGTCTGGCTGCAGTGTGTG(+ACTCAC)GATCCGGACATTGAGGTCACAGAACAGGAGC NGG 10 8 17 8 24 0 100 5 10
    240604211350_nftc GTGCTGCTCCTGTTCTGTGACCTCAATGTCCG(+ACTCAC)GATCCACACACTGCAGCCAGACCCCTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240604211348_702r CTTCCTGTACCTAAGGGCACTGGCTGGATTGG(+ACTCAC)GTACCGGGTAGCAGAAAAGAACGGGAGCCTG NGG 10 8 17 8 24 0 100 5 10