Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211346_bls5 GCAGGCAGGCTCCCGTTCTTTTCTGCTACCCG(+ACTCAC)GTACCCAATCCAGCCAGTGCCCTTAGGTACA NGG 10 8 17 8 24 0 100 5 10
    240604211343_7npl TCAAGCCTAGATTTGAGTTCTCCTTATCCCAG(+ACTCAC)GTACCTGGATTTATCCCTGCTGATTGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240604211341_u61j AACAGACTGACAATCAGCAGGGATAAATCCAG(+ACTCAC)GTACCTGGGATAAGGAGAACTCAAATCTAGG NGG 10 8 17 8 24 0 100 5 10
    240604211339_klax GACCCCGTGGGTGAACACGCACCAGGGTGGGG(+ACTCAC)GTACCAGTGCTCCAGTTGGCCTACTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604211337_ll2g GAAGGGGAAGAAGTAGGCCAACTGGAGCACTG(+ACTCAC)GTACCCCCACCCTGGTGCGTGTTCACCCACG NGG 10 8 17 8 24 0 100 5 10
    240604211336_hk8u TCTTGCTGAAATAAAGGTAAAGTCCACATTTG(+ACTCAC)GATCCTTTGCTCCTGCACCACTTTTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240604211335_65xr CCAGCCAATGGAAAAGTGGTGCAGGAGCAAAG(+ACTCAC)GATCCAAATGTGGACTTTACCTTTATTTCAG NGG 10 8 17 8 24 0 100 5 10
    240604211332_49fo TTTTTCTCTGAAATTCCTTGTCTCCGCCTCCG(+ACTCAC)GATCCAGGTCAGCAGCAGCCCCCAGAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240604211331_uawo CAGCCACAGCTCTGGGGGCTGCTGCTGACCTG(+ACTCAC)GATCCGGAGGCGGAGACAAGGAATTTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604211328_39vz TCAGCCTGAACCATTTCTCATCTGTTTCCCAG(+ACTCAC)GATCCAAAATTGTACCTGCCTTTCAATAACT NGG 10 8 17 8 24 0 100 5 10