Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211234_z4il TTTTTGGCAAAAAATTTTTACAACAAAATTGG(+ACTCAC)GATCCCATTGTATGAACTGAGTCGCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240604211233_svi1 TCCTCCGTGGAGCTGTCTCTAGTGAGAATCAG(+ACTCAC)GTACCTGATTTGGTGAACCTGGATGGGAACC NGG 10 8 17 8 24 0 100 5 10
    240604211231_kaf2 TCCCAGGTTCCCATCCAGGTTCACCAAATCAG(+ACTCAC)GTACCTGATTCTCACTAGAGACAGCTCCACG NGG 10 8 17 8 24 0 100 5 10
    240604211230_ec6e ATGGTGGACCGGCCACTGTGCCTATGCTATGG(+ACTCAC)GATCCCATCTCTTACAGCCCTGCAGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240604211228_q4go CAAGAGCTTCCCTGCAGGGCTGTAAGAGATGG(+ACTCAC)GATCCCATAGCATAGGCACAGTGGCCGGTCC NGG 10 8 17 8 24 0 100 5 10
    240604211226_pte8 TGTGGACCCCTACTGGGACGTGCTTGGTTTGG(+ACTCAC)GTACCTTCCTCGGTCCCACTGGCCGATTCTC NGG 10 8 17 8 24 0 100 5 10
    240604211225_2f17 AAGTTGAGAATCGGCCAGTGGGACCGAGGAAG(+ACTCAC)GTACCCAAACCAAGCACGTCCCAGTAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240604211223_3ses CCACCTTGAAATCCCAAAGAAAGCAGTTCTTG(+ACTCAC)GTACCATCTATTATGGTTCTACCATTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604211222_yld3 CCCCTTTTTTAATGGTAGAACCATAATAGATG(+ACTCAC)GTACCAAGAACTGCTTTCTTTGGGATTTCAA NGG 10 8 17 8 24 0 100 5 10
    240604211220_wkbr TCTCAATGCAGAATTACTGTGTGCAGGGGGAG(+ACTCAC)GTACCAGGTCCCTTTCATTTGGGCTCAACTC NGG 10 8 17 8 24 0 100 5 10