Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211252_dl7q CATGGACCTTTCTCAGGCTGGGGCAGGGTGGG(+ACTCAC)GATCCAGGCCTCAGTCACTGTCAGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240604211250_aewe ACATTCTCAGTTATTTCCTATTGGCAGCTGAG(+ACTCAC)GATCCACTGCAATCTGCATGTGATCATTGAA NGG 10 8 17 8 24 0 100 5 10
    240604211249_jf2m TACAATTCAATGATCACATGCAGATTGCAGTG(+ACTCAC)GATCCTCAGCTGCCAATAGGAAATAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604211247_5we5 TGGCCAAGCCCCTTCCCTTTCCTCAGTTTTAG(+ACTCAC)GATCCTTCATTCATATTAGAAAAAGGATCTG NGG 10 8 17 8 24 0 100 5 10
    240604211246_0zlp GCCCCCAGATCCTTTTTCTAATATGAATGAAG(+ACTCAC)GATCCTAAAACTGAGGAAAGGGAAGGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604211243_blcw GTTCAGCCTAGTCGGCCTACAAAGTTACCAAG(+ACTCAC)GATCCTCCTGCCTCATTCTCTCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604211242_wsnb GAATCCCAGCACTTGAGAGAATGAGGCAGGAG(+ACTCAC)GATCCTTGGTAACTTTGTAGGCCGACTAGGC NGG 10 8 17 8 24 0 100 5 10
    240604211239_pk2p GACTACTTATTCCCCAGATAATCATCTGGATG(+ACTCAC)GTACCTGGCAGCTGGCAGGGCACCACGAGAT NGG 10 8 17 8 24 0 100 5 10
    240604211238_jljh TGCTGATCTCGTGGTGCCCTGCCAGCTGCCAG(+ACTCAC)GTACCATCCAGATGATTATCTGGGGAATAAG NGG 10 8 17 8 24 0 100 5 10
    240604211236_5hzs TTTTCAGACCAGCGACTCAGTTCATACAATGG(+ACTCAC)GATCCCAATTTTGTTGTAAAAATTTTTTGCC NGG 10 8 17 8 24 0 100 5 10