Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211327_61an GGAGGAGTTATTGAAAGGCAGGTACAATTTTG(+ACTCAC)GATCCTGGGAAACAGATGAGAAATGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240604211325_q3s5 GAGGTCTTAAGATCCCATGGAGGGTCCTGTGG(+ACTCAC)GTACCTTGTGGGGGTCCGCAGACTCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604211323_8iit CCTTGGGCACAGAGTCTGCGGACCCCCACAAG(+ACTCAC)GTACCCACAGGACCCTCCATGGGATCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240604211321_yyf7 GGATGGACCATGTAGAGACTGCCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240604211319_eyjy GGTGTCAGCATTTGGAAGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604211317_y0mo CTTTAATGCCATCACTTGAGCAGAGACAGGTG(+ACTCAC)GATCCCTAAGTTTAAGTCCAGCCTCATCTAC NGG 10 8 17 8 24 0 100 5 10
    240604211314_c4w6 GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+ACTCAC)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604211313_hw9h CTTTAATGCCATCACTTGAGCAGAGACAGGTG(+ACTCAC)GATCCCTAAGTTTAAGTCCAGCCTCATCTAC NGG 10 8 17 8 24 0 100 5 10
    240604211311_zs05 GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+ACTCAC)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604211310_moun TAATGGATAAGGCTTTATTTCTCTGCTGGAAG(+ACTCAC)GATCCATCTGGGAGAAAGAGAAGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10