Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210600_r3tq AAGCTTTTAATCTCTTACTGGTCATTGGATGG(+ACTCAC)GATCCACCCTCAAACATGCATGAGGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604210559_gfag CCACCCCAGCCAAGAGCTGAGGCGCATGAGAG(+ACTCAC)GATCCTGAGCATCCCCAGAAGGGTGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604210558_2d7b CACAGCAGACACACCCTTCTGGGGATGCTCAG(+ACTCAC)GATCCTCTCATGCGCCTCAGCTCTTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604210556_flco CAGGTGGCTAACAACTGCCTTTAACTTCAAGG(+ACTCAC)GATCCAAAGCCCTCCTCAGGCACCTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240604210555_wfeh TATGTGGGTACAGGTGCCTGAGGAGGGCTTTG(+ACTCAC)GATCCCTTGAAGTTAAAGGCAGTTGTTAGCC NGG 10 8 17 8 24 0 100 5 10
    240604210553_k9r3 AAGTTCTTACAGTTTCCAGACTATAATTTTAG(+ACTCAC)GATCCAGAAAGGACTCGGAGAACGCATCCCC NGG 10 8 17 8 24 0 100 5 10
    240604210552_dmbw TTGTGGGGGATGCGTTCTCCGAGTCCTTTCTG(+ACTCAC)GATCCTAAAATTATAGTCTGGAAACTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240604210551_w2op AGCCTGTGAGTTATATCCCCCCAGCAGCCATG(+ACTCAC)GATCCCATGGCTTCAACAGCGGGTGTACTTG NGG 10 8 17 8 24 0 100 5 10
    240604210548_4jng ATACACAAGTACACCCGCTGTTGAAGCCATGG(+ACTCAC)GATCCATGGCTGCTGGGGGGATATAACTCAC NGG 10 8 17 8 24 0 100 5 10
    240604210547_h0k3 TCAAAAGACAGGATAAGGTGAAGCCAGAACGG(+ACTCAC)GATCCGCTCAGGAAGTCCTGGGGGCACTGGT NGG 10 8 17 8 24 0 100 5 10