Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210513_v5m6 GCTGGCTTGCTTCCTTTCTAAGCAATAACGTG(+ACTCAC)GATCCCGTTTTAGTCAAACTGACTGCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604210511_0vxj AGTATAGTATCAATAACGTCTACATCACCAGG(+ACTCAC)GTACCTTTGCATTAAATGAGTTTTGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604210509_2bkm TTACTATTTTGCAAAACTCATTTAATGCAAAG(+ACTCAC)GTACCCTGGTGATGTAGACGTTATTGATACT NGG 10 8 17 8 24 0 100 5 10
    240604210508_s104 CAAAGATTAAAGAGGACCAAAAGATGAGTTCG(+ACTCAC)GTACCATAACAAAAAAAATCTCACCTACTGT NGG 10 8 17 8 24 0 100 5 10
    240604210507_dkmn ATGGTACAGTAGGTGAGATTTTTTTTGTTATG(+ACTCAC)GTACCGAACTCATCTTTTGGTCCTCTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240604210506_ndo0 ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+ACTCAC)GATCCCCAGTTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604210504_ynqj GGAATGACCATCCAGAGACTGCTCTAACTGGG(+ACTCAC)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604210503_pzkv TTCTGGGTATGTGTCCAGGAGCATAATTGCTG(+ACTCAC)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604210501_1f51 TACTCAGAAAATAGTTCACAGTACTACAGGAG(+ACTCAC)GATCCAGCAATTATGCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240604210500_424n CACAATTTTCCTTTTCATCCCCCACCACATTG(+ACTCAC)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10