Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210545_sjvo GATGGACCAGTGCCCCCAGGACTTCCTGAGCG(+ACTCAC)GATCCCGTTCTGGCTTCACCTTATCCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604210543_8sw7 TGTCGACCCAGTACTGTGGGCAGAGGCAAGAG(+ACTCAC)GATCCCTGCAAAGTGGTGGCCAAACAGAACA NGG 10 8 17 8 24 0 100 5 10
    240604210542_n9fe GGTTTTGTTCTGTTTGGCCACCACTTTGCAGG(+ACTCAC)GATCCTCTTGCCTCTGCCCACAGTACTGGGT NGG 10 8 17 8 24 0 100 5 10
    240604210540_53yb TCTGAGGAGTGTTGATATTAATAGTACCACTG(+ACTCAC)GTACCCAAGTTGAGTAATCCTGGATTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240604210539_wtqe CATCTTCAAAAATCCAGGATTACTCAACTTGG(+ACTCAC)GTACCAGTGGTACTATTAATATCAACACTCC NGG 10 8 17 8 24 0 100 5 10
    240604210537_oq2o TGACGTAGAAATGGGACAGAAGCCACAGCAGG(+ACTCAC)GATCCCTGCCGGGCCTTATGGGTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240604210535_zap8 CCTTCCTAAGCACACCCATAAGGCCCGGCAGG(+ACTCAC)GATCCCTGCTGTGGCTTCTGTCCCATTTCTA NGG 10 8 17 8 24 0 100 5 10
    240604210534_q8e9 TCCAGCAATCCCTTTTGTTTACAATCACATAG(+ACTCAC)GTACCTCGTATGCACAAGTCCCAGGCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240604210533_l9cz GTTCCTGGAAGCCTGGGACTTGTGCATACGAG(+ACTCAC)GTACCTATGTGATTGTAAACAAAAGGGATTG NGG 10 8 17 8 24 0 100 5 10
    240604210531_4pmc CACAGGCAAGACCTCCTCCAACTCTACACCTG(+ACTCAC)GTACCCAGGGCCTGCAGCAGTGGCAGGATCT NGG 10 8 17 8 24 0 100 5 10