Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210458_1sad CCTCTACGAGAAAAGTGATGGTATATATTTAG(+ACTCAC)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604210457_gnoo ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+ACTCAC)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604210454_raa4 GGAATGACCATCCAGAGACTGCTCTACCTGGG(+ACTCAC)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604210453_1v8i TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+ACTCAC)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604210452_h291 TACTCAGAAAATAGTTCACAGTACTACAGGAG(+ACTCAC)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240604210451_zlnv CACAATTTTCCTTTTCATCCCCCACCACATTG(+ACTCAC)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240604210449_vykr CCTCTACGAGAAAAGTGATGGTATATATTTAG(+ACTCAC)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604210448_6p7v ACTGTCTGGGTTTGGTGACTGATGATGGAATG(+ACTCAC)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604210445_df3l GGAATGACCATCCAGAGACTGCTCTACCTGGG(+ACTCAC)GATCCATTCCATCATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604210444_68sc TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+ACTCAC)GATCCTCCTGTAGTACTATGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10