Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210442_4403 TACTCAGAAAATAGTTCATAGTACTACAGGAG(+ACTCAC)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240604210441_cfn9 CACAATTTTCCTTTTCATCCCCCACCACATTG(+ACTCAC)GATCCTAAATATATACCATCACTTTTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240604210440_u7yy CCTCTATGAGAAAAGTGATGGTATATATTTAG(+ACTCAC)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604210438_2jsu GTAAAAGGTCTACAATACCAGATGCGAGTCGG(+ACTCAC)GATCCAAATCACCCAGCCTAACTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240604210437_5gb4 TGTAGCTAAGCACAGTTAGGCTGGGTGATTTG(+ACTCAC)GATCCCGACTCGCATCTGGTATTGTAGACCT NGG 10 8 17 8 24 0 100 5 10
    240604210435_qu09 ATGGAGAGGGTGTGCACTAACTGAAACTTTTG(+ACTCAC)GATCCAAGGACAAGACAATCTTTTCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240604210432_25n4 AAACTGATAAGGAAAAGATTGTCTTGTCCTTG(+ACTCAC)GATCCAAAAGTTTCAGTTAGTGCACACCCTC NGG 10 8 17 8 24 0 100 5 10
    240604210431_8yku GTGCTAAAGAGATTTTCACAGACATCTTCCAG(+ACTCAC)GTACCTGGTCTAGTTAGTGGCAGGGATATGT NGG 10 8 17 8 24 0 100 5 10
    240604210429_x5jy GTAAAACATATCCCTGCCACTAACTAGACCAG(+ACTCAC)GTACCTGGAAGATGTCTGTGAAAATCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604210427_zx7c TCGGTGTGGAAAAAAGATGACCAGAAAGTCAG(+ACTCAC)GTACCCCGAATCACTGGAAACTCCTTTCTTA NGG 10 8 17 8 24 0 100 5 10