Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210329_65ew TTTCACATTGAGACTTGAATTAAATTTGATTG(+ACTCAC)GATCCTGTGTGAGGCAAGGGGCAAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604210327_xwh1 AATGAGTCAGAATTGGCCCTACAGCCCCTTTG(+ACTCAC)GATCCTGCAGGATACCCTAGTGGCTAAACCT NGG 10 8 17 8 24 0 100 5 10
    240604210325_qcgq GGGCTAGGTTTAGCCACTAGGGTATCCTGCAG(+ACTCAC)GATCCAAAGGGGCTGTAGGGCCAATTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240604210324_27x2 TGGGGCAGCTGTCAAAAGCTGCAGTTGGATAG(+ACTCAC)GTACCAGCCTGTGGTGACTGGTAAGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604210321_8nqb GTTTGCCACTACTTACCAGTCACCACAGGCTG(+ACTCAC)GTACCTATCCAACTGCAGCTTTTGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604210320_udu7 GTCCCAGTTTCCACCTTCCTCTGGCCCAGTAG(+ACTCAC)GATCCCCAATCTGACTCTTTCAAGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604210317_fb11 GAATGTCTGAGCCTTGAAAGAGTCAGATTGGG(+ACTCAC)GATCCTACTGGGCCAGAGGAAGGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240604210315_yaxe GGTAACCACATTTTTCAACTCCACCCACCATG(+ACTCAC)GTACCCTAGAACCAAGACTGGAATACTGACT NGG 10 8 17 8 24 0 100 5 10
    240604210313_wm1x TCTCCAGTCAGTATTCCAGTCTTGGTTCTAGG(+ACTCAC)GTACCATGGTGGGTGGAGTTGAAAAATGTGG NGG 10 8 17 8 24 0 100 5 10
    240604210312_hj10 ACCACATGGTGGCCCACAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10