Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210232_o6hb CACATTCCTGTCCTAAACCAGGTGAGGCCAGG(+ACTCAC)GTACCTCCAGCATCCTTCCTGCAGCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240604210231_xrsl ACGGCCCCAAGAAATGCTGAGATTTTACAATG(+ACTCAC)GATCCATACTCCATTAAGAGATGTGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604210229_nbhy GTATGAACTCACACATCTCTTAATGGAGTATG(+ACTCAC)GATCCATTGTAAAATCTCAGCATTTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604210227_b5n5 ACCCCCTGCAGTGGGCTAATTAGGACTGAGGG(+ACTCAC)GTACCTCTAAGGCTTTGGGGGTTCAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240604210226_s1cf GAGGCCTCTGTTGAACCCCCAAAGCCTTAGAG(+ACTCAC)GTACCCCTCAGTCCTAATTAGCCCACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240604210224_a4jq AGAAGGGGACCCTGGCCACAGGACACTTCCAG(+ACTCAC)GATCCATGCTCCCCTCCTCCTTGGGTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240604210222_5srz TCAAACTCAGACCCAAGGAGGAGGGGAGCATG(+ACTCAC)GATCCTGGAAGTGTCCTGTGGCCAGGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240604210220_reqy GCTCCTGAGACTTCCCACTTTTAGCAGTTGGG(+ACTCAC)GTACCAGGCCAGCAGCACCAGGGGCCAGATG NGG 10 8 17 8 24 0 100 5 10
    240604210217_x2ak CAACCCATCTGGCCCCTGGTGCTGCTGGCCTG(+ACTCAC)GTACCCCAACTGCTAAAAGTGGGAAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240604210216_km5q TGAGCCATGCCTCCAGGTAACTGATGGATTTG(+ACTCAC)GTACCTCTCATCCCAGGCTTTTTAAGAAAGC NGG 10 8 17 8 24 0 100 5 10