Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210215_o7qd CCAAGGCTTTCTTAAAAAGCCTGGGATGAGAG(+ACTCAC)GTACCAAATCCATCAGTTACCTGGAGGCATG NGG 10 8 17 8 24 0 100 5 10
    240604210213_0oe3 CTGACATCCAATGGGTCACACCCACAAGTTGG(+ACTCAC)GTACCCGTGAGTTCACTCAGGTGTGTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604210211_2prs GGCCTCAAAGACACACCTGAGTGAACTCACGG(+ACTCAC)GTACCCAACTTGTGGGTGTGACCCATTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604210210_u8k2 GTCTCCCACTCCTTGCTTGTTGCCTTTAGAAG(+ACTCAC)GTACCAGGTGGGACTGGGGCTGTGGGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240604210208_6c2x TCCACCTTGCCCCACAGCCCCAGTCCCACCTG(+ACTCAC)GTACCTTCTAAAGGCAACAAGCAAGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604210207_d3x9 AGGGGAGCTTGTGATTGCTATGCCTTCAACAG(+ACTCAC)GATCCCTCAGGGTCTCATCTTGAAGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604210205_3sag AGCCCTCCACTCTTCAAGATGAGACCCTGAGG(+ACTCAC)GATCCTGTTGAAGGCATAGCAATCACAAGCT NGG 10 8 17 8 24 0 100 5 10
    240604210203_4hjp GCTTACATTTACTGGCCCTGAAGCTTCCAAAG(+ACTCAC)GATCCGCATAAAAACCTAGAGAAAGAATAAG NGG 10 8 17 8 24 0 100 5 10
    240604210202_hjw5 CTTCCCTTATTCTTTCTCTAGGTTTTTATGCG(+ACTCAC)GATCCTTTGGAAGCTTCAGGGCCAGTAAATG NGG 10 8 17 8 24 0 100 5 10
    240604210200_w6um TCCTAGGAGGCCTTTGTTTTCCCAAATTCAAG(+ACTCAC)GATCCAAAAATTCTTCAGGTCAGAAAACTAA NGG 10 8 17 8 24 0 100 5 10