Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210252_pwat ATGCTCACAATCCCATTCAGAAGCTCTTATGG(+ACTCAC)GTACCCACAGCTATTTTCAAAGAAGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240604210249_jqpt GTTTGTAGAGGGACCTTTCTTCCCTTCCCCTG(+ACTCAC)GATCCCGAAGGTGACAGGCTGTTGTCTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604210247_m20s CCACCATCCAGACAACAGCCTGTCACCTTCGG(+ACTCAC)GATCCAGGGGAAGGGAAGAAAGGTCCCTCTA NGG 10 8 17 8 24 0 100 5 10
    240604210245_5mns TGGGAGTACAGAAGGAGCTGAAGGTATAGAGG(+ACTCAC)GATCCCAGACTGTGGCTGGACCTGGCTATGC NGG 10 8 17 8 24 0 100 5 10
    240604210243_6l48 TAGCTGCATAGCCAGGTCCAGCCACAGTCTGG(+ACTCAC)GATCCCTCTATACCTTCAGCTCCTTCTGTAC NGG 10 8 17 8 24 0 100 5 10
    240604210241_x901 CAGGTCTTCCCTAGAGTCCTAAGGGCATCGAG(+ACTCAC)GATCCCATCTGCCATGTTTCAGTCTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240604210240_duc0 CAGTGGAGGGCAGACTGAAACATGGCAGATGG(+ACTCAC)GATCCTCGATGCCCTTAGGACTCTAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604210238_8crc TGTATGATCTGGGACAGGCTGAAGCAGGCAGG(+ACTCAC)GTACCCACTCTGCAGTGTAGTTCCTGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240604210236_isjo CTTCTCGCTCCAGGAACTACACTGCAGAGTGG(+ACTCAC)GTACCCTGCCTGCTTCAGCCTGTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240604210235_bdui GTTACACCTAGGCTGCAGGAAGGATGCTGGAG(+ACTCAC)GTACCCTGGCCTCACCTGGTTTAGGACAGGA NGG 10 8 17 8 24 0 100 5 10