Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210005_f3pj TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+ACTCAC)GATCCAACTCACAATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240604210004_ha61 TATCTTGTGAACTGTCACTGCCATGGAGCAAG(+ACTCAC)GATCCCACCTCAGTGTTGAGACATCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240604210002_m7uk GACCCAGCTCTGATGTCTCAACACTGAGGTGG(+ACTCAC)GATCCTTGCTCCATGGCAGTGACAGTTCACA NGG 10 8 17 8 24 0 100 5 10
    240604210000_v3t6 ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+ACTCAC)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240604205959_auvy TTACACACTACCAAGTCCAGCCACAGCACAAG(+ACTCAC)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240604205958_69so TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+ACTCAC)GTACCTACTCAGTTATGGCAAGTAGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240604205956_aibs ATAAGTAACGTCTACTTGCCATAACTGAGTAG(+ACTCAC)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604205955_cpsg GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+ACTCAC)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240604205954_yu8v AATAGTTTTATTTCACACCTTGTTTCTAGCTG(+ACTCAC)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240604205952_g036 TCTTTTGGAGTTGTCCAAAAGATCGTGAGTTG(+ACTCAC)GATCCCAGACATTGGACAGTTGGAGTTTCAT NGG 10 8 17 8 24 0 100 5 10