Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205933_8wv3 GGACAGAGGAGCTGAGTGCCTCTGAGGGGCTG(+ACTCAC)GATCCTGGTGGGAGTGGCTGATAGAGAAACT NGG 10 8 17 8 24 0 100 5 10
    240604205930_95gh GAGAGGACTCTGAAGCATCCTAGCTGCCCTGG(+ACTCAC)GTACCCAGCAACACTGCTAAACGGCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604205928_iaxg GTTTGCCAGGAGCCGTTTAGCAGTGTTGCTGG(+ACTCAC)GTACCCAGGGCAGCTAGGATGCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240604205927_glva AGAGGCACTGCCTCTACACTCATAAATTTTTG(+ACTCAC)GTACCAAACTTCTTAAAAACCAGATGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604205926_kqli TTTTTTTAACCATCTGGTTTTTAAGAAGTTTG(+ACTCAC)GTACCAAAAATTTATGAGTGTAGAGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240604205925_uztm TTTAAAATATAGGTTACCCTGCTACCCTGCTG(+ACTCAC)GATCCTGAGCCTGGAGGCCTGCAGATCCATC NGG 10 8 17 8 24 0 100 5 10
    240604205923_g7db GCCCGGATGGATCTGCAGGCCTCCAGGCTCAG(+ACTCAC)GATCCAGCAGGGTAGCAGGGTAACCTATATT NGG 10 8 17 8 24 0 100 5 10
    240604205921_z0ie GGCAACTGGGGGGAGGTGGGGTCAGGAACCCG(+ACTCAC)GATCCACCAAGAGGACAGTTCCCAAAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240604205918_k2q5 CTCCCTCCTCTTTGGGAACTGTCCTCTTGGTG(+ACTCAC)GATCCGGGTTCCTGACCCCACCTCCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604205917_08i4 GTGGAGGTGTCTTTAACAAACACTTTACTTTG(+ACTCAC)GTACCAGCACTCAGGTGACACGGCTCAGAGG NGG 10 8 17 8 24 0 100 5 10