Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205855_rzqx TGGATCATCACCCCCGCTGCTCTCCCATTCCG(+ACTCAC)GTACCTAAGTAACATCTATGCAAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240604205854_nt9n TGTGATACCAGCATGCCCTGATACGCCGTTAG(+ACTCAC)GTACCACAGGTCTACTACTGGGAGAGCTATG NGG 10 8 17 8 24 0 100 5 10
    240604205852_fxq7 AGGGTCATAGCTCTCCCAGTAGTAGACCTGTG(+ACTCAC)GTACCTAACGGCGTATCAGGGCATGCTGGTA NGG 10 8 17 8 24 0 100 5 10
    240604205849_lwbb ACTTTCTATTCCAACCTCCCTCTACACCAGCG(+ACTCAC)GTACCCAACCTTCCTCATGCTGCCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240604205848_fc4z GTACTAAAGGGTGGCAGCATGAGGAAGGTTGG(+ACTCAC)GTACCGCTGGTGTAGAGGGAGGTTGGAATAG NGG 10 8 17 8 24 0 100 5 10
    240604205845_19ke GAGAGAGAGGGGTGGACTGAACATGGCCGGGG(+ACTCAC)GATCCGTGAGACGGAGAGGAAACTGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240604205843_a1vz CTATCCTGTGCCAGTTTCCTCTCCGTCTCACG(+ACTCAC)GATCCCCCGGCCATGTTCAGTCCACCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240604205841_0x4o TTAAAGAGTAACATCTACCTTGCAGTGGCCAG(+ACTCAC)GATCCTAGGACCTCTCACTGACTAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240604205839_9qp6 ATCAAAGCGCTCTAGTCAGTGAGAGGTCCTAG(+ACTCAC)GATCCTGGCCACTGCAAGGTAGATGTTACTC NGG 10 8 17 8 24 0 100 5 10
    240604205837_qmo3 GCCAGGATCTCTGCTTTTTCCCTTTGCATCCG(+ACTCAC)GTACCCCTAACAAAGATTTGTTTCTGCACTA NGG 10 8 17 8 24 0 100 5 10