Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205916_10zl AAGGGCCTCTGAGCCGTGTCACCTGAGTGCTG(+ACTCAC)GTACCAAAGTAAAGTGTTTGTTAAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240604205914_jaob TCACCATTCCTAACAGAAGCACTGCACTGTTG(+ACTCAC)GTACCGAGAAGGGTGGAAGACAGGAGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604205913_rl3e CCCCCGCCCCCTCCTGTCTTCCACCCTTCTCG(+ACTCAC)GTACCAACAGTGCAGTGCTTCTGTTAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240604205906_8yvw ACGGCTCTTTAGGACAACACCACAAACCCAGG(+ACTCAC)GATCCCCAGTGGGGAGGAGGGAACAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240604205904_ni93 CATAAAAGATCTGTTCCCTCCTCCCCACTGGG(+ACTCAC)GATCCCTGGGTTTGTGGTGTTGTCCTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604205902_h9c9 ACCAGGACAATGTCACCTCAGGATGGGACTGG(+ACTCAC)GATCCCTGCAAGATGGTTCTACATGGAACCC NGG 10 8 17 8 24 0 100 5 10
    240604205900_c4qz GAGCCGGGTTCCATGTAGAACCATCTTGCAGG(+ACTCAC)GATCCCAGTCCCATCCTGAGGTGACATTGTC NGG 10 8 17 8 24 0 100 5 10
    240604205859_chfb TTTCCAGTCGTCTGAAGCCCGTGCGGGATTTG(+ACTCAC)GATCCTCAGCCACTGCTACGGAGCCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604205858_wp88 GACAAGGCACAGGCTCCGTAGCAGTGGCTGAG(+ACTCAC)GATCCAAATCCCGCACGGGCTTCAGACGACT NGG 10 8 17 8 24 0 100 5 10
    240604205856_4n12 TTAATGTGTTTCTTTGCATAGATGTTACTTAG(+ACTCAC)GTACCGGAATGGGAGAGCAGCGGGGGTGATG NGG 10 8 17 8 24 0 100 5 10