Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193335_i720 CTTATGCTAGGGAAGAGCTCTGCCATTGAATG(+ACTCAC)GTACCTCTGGCTCCCCAGCAAGCTCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240604193334_7ht6 TCCGATGTCTGATGTGTCATCTTTTTATCTTG(+ACTCAC)GATCCTTCCCTGGTTTGAAACTTTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240604193332_qi54 TTTCAAGAGTTTAAAGTTTCAAACCAGGGAAG(+ACTCAC)GATCCAAGATAAAAAGATGACACATCAGACA NGG 10 8 17 8 24 0 100 5 10
    240604193331_8k6d CCTGTCCTTTAATTCTCAGAACCCCTTTCGAG(+ACTCAC)GTACCTACATTGACTGGTAGTCGAGGGTCGA NGG 10 8 17 8 24 0 100 5 10
    240604193330_0ncv TGTAGTCGACCCTCGACTACCAGTCAATGTAG(+ACTCAC)GTACCTCGAAAGGGGTTCTGAGAATTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604193329_n9jt TGCTTCTGCCAAATGACTAATATGGCCTGCTG(+ACTCAC)GATCCCGAGCACACGGGGGCTTTGAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240604193327_irc4 TGAGTGTGACCTCAAAGCCCCCGTGTGCTCGG(+ACTCAC)GATCCAGCAGGCCATATTAGTCATTTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604193326_qwuf TTAACTTTTCCCAGGGGTCCAGCACCTCACCG(+ACTCAC)GATCCACCTAAGGGCGCTTACTAAAAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604193324_73f3 AGATGCCACCTTTTAGTAAGCGCCCTTAGGTG(+ACTCAC)GATCCGGTGAGGTGCTGGACCCCTGGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604193322_es8o AAGAAATCAGCAATCAGTAGTCCTCCCCTGAG(+ACTCAC)GATCCTCGGAACCTCCCTCTAGGCAAATCAA NGG 10 8 17 8 24 0 100 5 10