Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193253_cvr6 TTGTTACACATGACCTTTGACACCCATTTGTG(+ACTCAC)GTACCAAGTTTTCCAAGCAGAGCCTGAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604193252_6juw CTGGCCCCAGGCTGGGGTCCTATGCAAAGCAG(+ACTCAC)GATCCCACTAATGGGATTCAGGCAGAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240604193250_qf7s GGTAGACACTTCTGCCTGAATCCCATTAGTGG(+ACTCAC)GATCCTGCTTTGCATAGGACCCCAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604193248_5tik CGACTCAAAGGGCCTTCTTGCTGGGGCCTCTG(+ACTCAC)GATCCTCTGTCCTGGGGCCGGAAGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240604193247_180n TCCGGCCCCTGCCTTCCGGCCCCAGGACAGAG(+ACTCAC)GATCCAGAGGCCCCAGCAAGAAGGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604193246_h3zb TTGTCATTCATCACGAAGTAGGCCAAATCATG(+ACTCAC)GTACCACAGGAATTTAGAAATCTAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240604193244_czd0 CCAGATTGCAGTTAGATTTCTAAATTCCTGTG(+ACTCAC)GTACCATGATTTGGCCTACTTCGTGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240604193243_689v AAAGCCCTGGGTTGACTGACTCCCTCACTCAG(+ACTCAC)GATCCTAAGAACAATGCTGACTGCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604193242_5723 AAGTAGAAAACTGCAGTCAGCATTGTTCTTAG(+ACTCAC)GATCCTGAGTGAGGGAGTCAGTCAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604193240_bjrc GTGCAAGCGCCATGGCACCCTGCTGTACCACG(+ACTCAC)GTACCAAACTCAACTGTACAGTCAACAGTCA NGG 10 8 17 8 24 0 100 5 10