Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193239_dlq8 CTCGCTGACTGTTGACTGTACAGTTGAGTTTG(+ACTCAC)GTACCGTGGTACAGCAGGGTGCCATGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240604193237_5sf1 CGTAGGCCGATGAGTGTAAGTAAAAGAGTGTG(+ACTCAC)GTACCACTGGAAAAAACTCCAAACCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604193235_5f3u TCGATGGCCCTGGTTTGGAGTTTTTTCCAGTG(+ACTCAC)GTACCACACTCTTTTACTTACACTCATCGGC NGG 10 8 17 8 24 0 100 5 10
    240604193233_7am4 AGAAGGCCCCTCTGAGTATTTATTTGACCGGG(+ACTCAC)GTACCAATGGTACAGCTATATATATGTACAG NGG 10 8 17 8 24 0 100 5 10
    240604193231_jjva TTACTCTGTACATATATATAGCTGTACCATTG(+ACTCAC)GTACCCCGGTCAAATAAATACTCAGAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604193230_wuur AAGGACAGCTAGGCCAGGGGCCTTTGTCTGTG(+ACTCAC)GTACCTAAGGAACCCTGAGTCAAACCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240604193228_1v4u GGCGAGGACTGGTTTGACTCAGGGTTCCTTAG(+ACTCAC)GTACCACAGACAAAGGCCCCTGGCCTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604193227_xc95 CGGGAGATATGAAGCCAAGCTGTGTAGTTCAG(+ACTCAC)GATCCCGAGACCCTAAACACCACACTGACCT NGG 10 8 17 8 24 0 100 5 10
    240604193225_1vpm AAAGAAGGTCAGTGTGGTGTTTAGGGTCTCGG(+ACTCAC)GATCCTGAACTACACAGCTTGGCTTCATATC NGG 10 8 17 8 24 0 100 5 10
    240604193224_rfdc TCCATCCAAGTGTGGAAGGACCTCCTCTGAGG(+ACTCAC)GATCCACTGTAGCGAATCCATCCCAACCCTC NGG 10 8 17 8 24 0 100 5 10