Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193307_ff4i GGAGCTGAGTGAGGAAGCATACACTCAAACAG(+ACTCAC)GATCCTTCCAAGCCATGTGCAGCCTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240604193306_wq0w ACTAGGAACAACTAGTTGAGCAAGTCTTTAAG(+ACTCAC)GTACCGATTGTCAAAATACATCACTAAGACA NGG 10 8 17 8 24 0 100 5 10
    240604193305_khh0 TAATTTGTCTTAGTGATGTATTTTGACAATCG(+ACTCAC)GTACCTTAAAGACTTGCTCAACTAGTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240604193303_ct6c AATGCCTTCTTCAGTTTACCAGCAGTGGTGTG(+ACTCAC)GTACCAGTGAGGTATTTCAGGTAACGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604193302_x7w9 AAGAGAGAGACGTTACCTGAAATACCTCACTG(+ACTCAC)GTACCACACCACTGCTGGTAAACTGAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604193300_2ddo TTGGTACTATGATATCAAAATCAGAGGGAGGG(+ACTCAC)GATCCAGCTACCAGAACGGGAGTGGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240604193259_xaii CCTCCCTGAACCCACTCCCGTTCTGGTAGCTG(+ACTCAC)GATCCCCTCCCTCTGATTTTGATATCATAGT NGG 10 8 17 8 24 0 100 5 10
    240604193257_imzo TTGGACAGCCAGGGCTACACAGAGAGATCCTG(+ACTCAC)GATCCCGTCTTCCAAAAAAAAAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604193256_oceh CTTTCTTTCTTTTTTTTTTTTTTGGAAGACGG(+ACTCAC)GATCCAGGATCTCTCTGTGTAGCCCTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604193254_de48 TCAGAAACTTCAGGCTCTGCTTGGAAAACTTG(+ACTCAC)GTACCACAAATGGGTGTCAAAGGTCATGTGT NGG 10 8 17 8 24 0 100 5 10