Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192355_rz62 TCCATCCCCTTCCAGTTAAAACAGGAGAGGTG(+ACTCAC)GTACCAATACTCCTGGCGCTGGGCGTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240604192352_3zo2 GGTGTGTTTCCAGGAGCCAGCACCCCACCAGG(+ACTCAC)GTACCGCATAAACATTGCAGGAGAGTTTCCG NGG 10 8 17 8 24 0 100 5 10
    240604192351_mqe4 GGCTGCGGAAACTCTCCTGCAATGTTTATGCG(+ACTCAC)GTACCCTGGTGGGGTGCTGGCTCCTGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604192350_wnsd GTCAGGCTTTTCCCTCAGAAGATCACAGTTTG(+ACTCAC)GATCCTGAGAGGAATCTGCATGGGCTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240604192349_wcus GAGAGGCTGAAGCCCATGCAGATTCCTCTCAG(+ACTCAC)GATCCAAACTGTGATCTTCTGAGGGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604192347_2g9m GAGGCAACTTATTCCACCTTTTGGGTGCTGTG(+ACTCAC)GATCCCCCGGCTCCCCTCAGTCCCCTAAGCT NGG 10 8 17 8 24 0 100 5 10
    240604192345_snsc AAACAAGCTTAGGGGACTGAGGGGAGCCGGGG(+ACTCAC)GATCCACAGCACCCAAAAGGTGGAATAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604192343_ytgm AAAGGGGTTTTGATAGAAGGAAGAGACTTCCG(+ACTCAC)GTACCATTGTAACTAAAGTGTCAGTCATTTT NGG 10 8 17 8 24 0 100 5 10
    240604192342_d9uh TAGTTAAAATGACTGACACTTTAGTTACAATG(+ACTCAC)GTACCGGAAGTCTCTTCCTTCTATCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240604192340_udk1 ACATCTCCTGCCTCAGTCTTCAAAGCTATTTG(+ACTCAC)GATCCATAAGTTTGTTATATGCATCTTCTTG NGG 10 8 17 8 24 0 100 5 10