Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192323_gt7n TCATCCTATAGCTCAGGCTTCAAACTCACTGG(+ACTCAC)GATCCGCCTGCTCTGCCTCCTTAGTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604192321_rd6y GGACAGAAGCAGTTTGAGCAGGTGTCTATATG(+ACTCAC)GTACCCTGCATCCGGGGCCCTTTGGGAACTG NGG 10 8 17 8 24 0 100 5 10
    240604192320_lpqj CCGGCCAGTTCCCAAAGGGCCCCGGATGCAGG(+ACTCAC)GTACCATATAGACACCTGCTCAAACTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604192319_axd5 CACATTTCTAAAAATCTTTCAATGACAAGTTG(+ACTCAC)GTACCCTTTAGCTAACTAAAGCTAAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240604192317_xaws CCTTCCACCTTTTAGCTTTAGTTAGCTAAAGG(+ACTCAC)GTACCAACTTGTCATTGAAAGATTTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240604192316_m82g TCCTCCTATTCCTCCTGTGAGTTAGTGGCAGG(+ACTCAC)GTACCAACCCACTGGAGGGCAACAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240604192314_zwvz CCACACCCTCCCTGTTGCCCTCCAGTGGGTTG(+ACTCAC)GTACCCTGCCACTAACTCACAGGAGGAATAG NGG 10 8 17 8 24 0 100 5 10
    240604192312_z3wv GAAGGTCAGACAACAGCTTCCCTACTTGCCAG(+ACTCAC)GTACCTGCACTGGGGGGCCCCGGGCCACAGG NGG 10 8 17 8 24 0 100 5 10
    240604192311_kdjw CCAATCCTGTGGCCCGGGGCCCCCCAGTGCAG(+ACTCAC)GTACCTGGCAAGTAGGGAAGCTGTTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240604192310_k07u ATCCTGCCATGTGGCTGGTTACCTGTGCTCAG(+ACTCAC)GTACCATCCGTCCATCTCCTCACATCTTCCC NGG 10 8 17 8 24 0 100 5 10