Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192756_t6ok ATGAGGAATTCTCATGGTTCACACAGAACACG(+ACTCAC)GATCCTCAGGCAAGGCCGCGAAGAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240604192752_uq6e TTCTGGCAGACCAGACGACCAGTTAGCCCTGG(+ACTCAC)GATCCGTTTCTGTGCCCTGCATCCCGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604192751_rqe0 CTGTGGGGAACGGGATGCAGGGCACAGAAACG(+ACTCAC)GATCCCAGGGCTAACTGGTCGTCTGGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604192749_yloq GAAGTACCTGCAATATCAGCTCACTAACACGG(+ACTCAC)GTACCACTAGTTCTTGTTTCTCTTTGGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604192748_z3pp AAAATGACACCAAAGAGAAACAAGAACTAGTG(+ACTCAC)GTACCCGTGTTAGTGAGCTGATATTGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240604192744_6f7w ACTGCAACATGAGTTGGAGGGAAGAGCCCTGG(+ACTCAC)GTACCTGCCTGGTCCCCTCTGCTTCTATGAA NGG 10 8 17 8 24 0 100 5 10
    240604192721_i3ng TGGAGTTCATAGAAGCAGAGGGGACCAGGCAG(+ACTCAC)GTACCCAGGGCTCTTCCCTCCAACTCATGTT NGG 10 8 17 8 24 0 100 5 10
    240604192719_zr0q CATATGGGCTATGCAGAGAGATAGGACTTAGG(+ACTCAC)GTACCAGAACTTCCTGCTCTGTGGTCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604192714_qw76 GGATACCTGGGACCACAGAGCAGGAAGTTCTG(+ACTCAC)GTACCCTAAGTCCTATCTCTCTGCATAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604192712_78mk TTCCCCCTACACTCATTCTGCTTCCCCAGAAG(+ACTCAC)GATCCTAACACAGAAGATCCCACTCTAGCAC NGG 10 8 17 8 24 0 100 5 10