Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192338_w9nj ACATGCAAGAAGATGCATATAACAAACTTATG(+ACTCAC)GATCCAAATAGCTTTGAAGACTGAGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604192337_t5l4 CAAGGTAATGAGTTTCGTGGTAGCATTTTGAG(+ACTCAC)GATCCATGTCATCGTTCATTGTTCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240604192336_5sct GGGCTAGGAAAAGAACAATGAACGATGACATG(+ACTCAC)GATCCTCAAAATGCTACCACGAAACTCATTA NGG 10 8 17 8 24 0 100 5 10
    240604192334_v4fv CTTGCAAAAGCCAAAGCTGCCTGAGTAGTCTG(+ACTCAC)GATCCTGCAACATCACTTTGCTTCAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604192333_byek AAACTAACTCCTGAAGCAAAGTGATGTTGCAG(+ACTCAC)GATCCAGACTACTCAGGCAGCTTTGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240604192331_9mqd CTCCAAAGCTAGAGTAATCATGAGGGAGGCAG(+ACTCAC)GATCCACGGAGAATCACAAAGGATGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240604192330_xp0t GTATCAAAATGCATCCTTTGTGATTCTCCGTG(+ACTCAC)GATCCTGCCTCCCTCATGATTACTCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604192328_5zs7 GTATGCTGAGGTGTGGGGGTGGTGAATGAAGG(+ACTCAC)GTACCCTACCTGCAGTGCTCAAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240604192326_kgoi ATCATTGGACATTCTTGAGCACTGCAGGTAGG(+ACTCAC)GTACCCTTCATTCACCACCCCCACACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604192325_mw53 TTAATCCCAGCACTAAGGAGGCAGAGCAGGCG(+ACTCAC)GATCCCAGTGAGTTTGAAGCCTGAGCTATAG NGG 10 8 17 8 24 0 100 5 10