Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192254_f8ya ACCCCAAGCCTTTAACAACCAGAGTATAGCTG(+ACTCAC)GTACCTGACCCTACTGAGCCTGAAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604192252_fw6u ATAACTGGCTGGGGGGTTAGGCAAAGGACCTG(+ACTCAC)GTACCCTTACTACTCAAAGCCTACCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604192250_nuyx ACTGCCCACTAGGTAGGCTTTGAGTAGTAAGG(+ACTCAC)GTACCAGGTCCTTTGCCTAACCCCCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240604192248_dxmv ACCACGGCCTCTGAATGGGGCTGAGGCTCATG(+ACTCAC)GATCCCGGAATGAGACCAGGATGCATAAGAC NGG 10 8 17 8 24 0 100 5 10
    240604192246_40r0 CACTGGTCTTATGCATCCTGGTCTCATTCCGG(+ACTCAC)GATCCATGAGCCTCAGCCCCATTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240604192244_tiqh GGCTTCTTGTGATCTCCTTTGCCAGTCACTGG(+ACTCAC)GATCCAGAACAATCTACTTCTTCAAGCATGC NGG 10 8 17 8 24 0 100 5 10
    240604192243_f6p4 CAATGGCATGCTTGAAGAAGTAGATTGTTCTG(+ACTCAC)GATCCCAGTGACTGGCAAAGGAGATCACAAG NGG 10 8 17 8 24 0 100 5 10
    240604192241_mpld GGGTGACTGGATAACCCCAGCATGCCCAGCGG(+ACTCAC)GTACCATAAGCCCACACTGCTGGGATGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604192239_0rox CTGGGACCTCATCCCAGCAGTGTGGGCTTATG(+ACTCAC)GTACCCGCTGGGCATGCTGGGGTTATCCAGT NGG 10 8 17 8 24 0 100 5 10
    240604192237_30nm GATGGATAGGATGGATGCACCAACAGCTGTTG(+ACTCAC)GTACCTTGTTTTTCCTTCAACTGTTGGGTAT NGG 10 8 17 8 24 0 100 5 10