Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145011_x4dp TGAACCTAAGGCACAATAGGAAAGGGAGGTGG(+AGGACA)GTACCAGTGTGGGTCACAGCCTGAAGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613145010_islk GGGGTACAGGCTTCAGGCTGTGACCCACACTG(+AGGACA)GTACCCACCTCCCTTTCCTATTGTGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240613145009_v6b2 GGGGATCCAGCATCCTCCTCTGAGCTACTTTG(+AGGACA)GTACCACATACAGAAGTGTGCATATACATGC NGG 10 8 17 8 24 0 100 5 10
    240613145007_s4eu TTCTTGCATGTATATGCACACTTCTGTATGTG(+AGGACA)GTACCAAAGTAGCTCAGAGGAGGATGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613145005_f6re CATCACAAACATGGGAAACTCTGGTCCCAGGG(+AGGACA)GATCCAGCATCCTCCTCTGAGCTACTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613145003_3za2 GTGGTACCAAAGTAGCTCAGAGGAGGATGCTG(+AGGACA)GATCCCCTGGGACCAGAGTTTCCCATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613145002_sm1q AGAGGCAGACACAGGGAGCAGCTCCAGGAACG(+AGGACA)GTACCGTGGAGGATTCTGGCCAGGGCATCCC NGG 10 8 17 8 24 0 100 5 10
    240613145000_qmrw ACCCTGGGATGCCCTGGCCAGAATCCTCCACG(+AGGACA)GTACCGTTCCTGGAGCTGCTCCCTGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613144959_ba0p ATAAATAAAAACTCAAGGAAAATAAATACATG(+AGGACA)GATCCTATGCGGTTGGAGGGCGCGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613144957_nwf9 CTACATCCAGCCCGCGCCCTCCAACCGCATAG(+AGGACA)GATCCATGTATTTATTTTCCTTGAGTTTTTA NGG 10 8 17 8 24 0 100 5 10