Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144936_85ev TAAACAGCATAAGCGGATAGATTCTACTTGGG(+AGGACA)GATCCTGATGTCAAAGTAAGGACACACGGGA NGG 10 8 17 8 24 0 100 5 10
    240613144935_7t72 CACCCTCCCGTGTGTCCTTACTTTGACATCAG(+AGGACA)GATCCCCAAGTAGAATCTATCCGCTTATGCT NGG 10 8 17 8 24 0 100 5 10
    240613144933_aqnv ATGACCTGCTGATCCATGGGCTGACAACTTCG(+AGGACA)GTACCAGGTTCACACTCTTTGGCCATGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613144932_qnl6 GCGGGACTTCATGGCCAAAGAGTGTGAACCTG(+AGGACA)GTACCGAAGTTGTCAGCCCATGGATCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613144931_65bd AAAGGAAAAAAAAAGTGTGGGTGTGATGTCTG(+AGGACA)GATCCGTGTTTGCCTGCAGTAGCCTGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613144929_p844 GGATGTCTCACAGGCTACTGCAGGCAAACACG(+AGGACA)GATCCAGACATCACACCCACACTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613144927_5gba GGTTTGAGAAGCCCAATCTTGTCGGAGACGGG(+AGGACA)GATCCTCATTGATGGGAGGACTGAGGCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613144926_qx2p CCACTGCAGGCCTCAGTCCTCCCATCAATGAG(+AGGACA)GATCCCCGTCTCCGACAAGATTGGGCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613144925_mdhp GTCCTCTACCCCCTGTGATGTTGAGCGTGCTG(+AGGACA)GATCCTGGGGGCTGTCGGAGGCTGGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240613144922_biri CTGGCGCTGAACCAGCCTCCGACAGCCCCCAG(+AGGACA)GATCCAGCACGCTCAACATCACAGGGGGTAG NGG 10 8 17 8 24 0 100 5 10