Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145103_j48k CATTGAAAGAAGATGAAACGATCATTTCTAGG(+AGGACA)GTACCATGCAGTTATAAAGTACCAACACTCG NGG 10 8 17 8 24 0 100 5 10
    240613145101_adla ATAATTCATGGGGGGGGGGGGGCGCGCTAGGG(+AGGACA)GTACCATGATGGATGTGTGGAGGTCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613145100_azql AGTCATCCATTGACCTCCACACATCCATCATG(+AGGACA)GTACCCCTAGCGCGCCCCCCCCCCCCCATGA NGG 10 8 17 8 24 0 100 5 10
    240613145058_bwr3 GGAAAGGCAGTGGAGGAACTGGGGAGCTCTGG(+AGGACA)GTACCCAGAATCCTCGAGGTCTCATCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240613145056_b718 CAGGTGTCAAGATGAGACCTCGAGGATTCTGG(+AGGACA)GTACCCAGAGCTCCCCAGTTCCTCCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240613145055_ljq9 TGCCAAGAATCACATAGCTGAGGCTATAGATG(+AGGACA)GTACCGATGACTCTGTGGAACCGTGGTTACA NGG 10 8 17 8 24 0 100 5 10
    240613145054_lfro TTTATTGTAACCACGGTTCCACAGAGTCATCG(+AGGACA)GTACCATCTATAGCCTCAGCTATGTGATTCT NGG 10 8 17 8 24 0 100 5 10
    240613145052_t2tz TACGATGGAAATGTATCCTGCCACCTAGCTGG(+AGGACA)GTACCCATATGGACAGCAACCCAAGGACCCA NGG 10 8 17 8 24 0 100 5 10
    240613145050_jkn5 TTGTTTGGGTCCTTGGGTTGCTGTCCATATGG(+AGGACA)GTACCCAGCTAGGTGGCAGGATACATTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613145049_2vnw GCACCTTGACAAAATCAGGGTGTCTAGGGCTG(+AGGACA)GATCCCCTGAAGCTCTAAGGGAAACTCCATT NGG 10 8 17 8 24 0 100 5 10