Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144811_1ptz ACAGCTCTGCTAAGCCTGTTGCCCAGTACACG(+AGGACA)GTACCGGGAGCTCTTGTCCCTTTCCTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613144809_u30z TACCATGGAAAGGAAAGGGACAAGAGCTCCCG(+AGGACA)GTACCGTGTACTGGGCAACAGGCTTAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613144808_e162 GGTCAGCTGTAGCCGACCTGGGAAAGCTAGTG(+AGGACA)GTACCACACCATGTACTGAAAACCTGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613144807_rpli TGATAAGGAACAGGTTTTCAGTACATGGTGTG(+AGGACA)GTACCACTAGCTTTCCCAGGTCGGCTACAGC NGG 10 8 17 8 24 0 100 5 10
    240613144805_msqy GTTTAAACACTGGGTTAAGATCCTTAATTAAG(+AGGACA)GTACCCATGTCACGTTGACCCTCCATGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613144804_zi67 TTAAATCCACATGGAGGGTCAACGTGACATGG(+AGGACA)GTACCTTAATTAAGGATCTTAACCCAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613144803_v2hh AAACACTGATTATTACAAAGTTCATGGATATG(+AGGACA)GATCCCAGATGTGTGCAACGTGATGCCCGTG NGG 10 8 17 8 24 0 100 5 10
    240613144801_hjn6 CAAAGCACGGGCATCACGTTGCACACATCTGG(+AGGACA)GATCCATATCCATGAACTTTGTAATAATCAG NGG 10 8 17 8 24 0 100 5 10
    240613144759_juz0 CTATGAAAGGAATCGCCTCCATGGGGCCACAG(+AGGACA)GTACCTGAGCCTCTCACTTTGTCCGCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613144758_jh53 AAAAAAAGAGGCGGACAAAGTGAGAGGCTCAG(+AGGACA)GTACCTGTGGCCCCATGGAGGCGATTCCTTT NGG 10 8 17 8 24 0 100 5 10