Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144851_pnzz TGGTAATGGCACTGGGGAGGCTGGGGCAGGAG(+AGGACA)GATCCAGAATGCCAGGCCAGCTTGGACCAGA NGG 10 8 17 8 24 0 100 5 10
    240613144848_7yft CAGAGCAAACATGATGGCCTGCCATCTCCTGG(+AGGACA)GATCCCCCAAGGGTGACTTCCACTCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613144846_v8ry GAAAACCAGAGGAGTGGAAGTCACCCTTGGGG(+AGGACA)GATCCCAGGAGATGGCAGGCCATCATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613144845_p8jy ATTTCCAAGAAGGAAAGGTCGGCCTTTGTGCG(+AGGACA)GTACCACAGCCTCAGTTTCCTAGTCTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613144844_s2fg TTCACCCTGCAGACTAGGAAACTGAGGCTGTG(+AGGACA)GTACCGCACAAAGGCCGACCTTTCCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613144842_xr8r TCTAAAAGATGAAATATCCACTATCCAAAGGG(+AGGACA)GATCCTCAGAGGGCAACATAGTCAGCGACCA NGG 10 8 17 8 24 0 100 5 10
    240613144840_so5p AGGACTTTGACTAGCTGGAGAGGTCTGGGGAG(+AGGACA)GATCCAGGGCAGCACAAATGGGATGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613144838_nh0d CTTTGCAGCTTCATCCCATTTGTGCTGCCCTG(+AGGACA)GATCCTCCCCAGACCTCTCCAGCTAGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613144836_qcj0 TCACTGGCCAGTTAGCCTAAGCAAAGTAAATG(+AGGACA)GTACCTAAAAGAACACATAAGGTTCCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613144835_kj0s GGGCCAGAGGGGAACCTTATGTGTTCTTTTAG(+AGGACA)GTACCATTTACTTTGCTTAGGCTAACTGGCC NGG 10 8 17 8 24 0 100 5 10