Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144713_fbop TTTCGGGGTCTCTGTTTCTTTGTCTATAAAAG(+AGGACA)GATCCTTTACTAGGATAGAAAGTTGTGATAT NGG 10 8 17 8 24 0 100 5 10
    240613144712_cbg8 AAAACATATCACAACTTTCTATCCTAGTAAAG(+AGGACA)GATCCTTTTATAGACAAAGAAACAGAGACCC NGG 10 8 17 8 24 0 100 5 10
    240613144710_oqvt GGAAGGCCATGCTGTCCACCCTTCCTGGCCAG(+AGGACA)GATCCAGTTGAGCAGCAGAGGTTGACTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613144709_707x CCTTCATTTAGTCAACCTCTGCTGCTCAACTG(+AGGACA)GATCCTGGCCAGGAAGGGTGGACAGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240613144708_wfqp TATGGTTAGGTCTTCAGAACTTAATTAAATTG(+AGGACA)GATCCTGGAGATGAGGCACATGGGTAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613144706_pm6z CTACTTTCTCTACCCATGTGCCTCATCTCCAG(+AGGACA)GATCCAATTTAATTAAGTTCTGAAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240613144704_0ons GCTACCCTTAACTTTCACAGATCTAATAATCG(+AGGACA)GATCCCTAAAATAAACTTGAGGCACATTTTG NGG 10 8 17 8 24 0 100 5 10
    240613144703_wuap ATGTTCAAAATGTGCCTCAAGTTTATTTTAGG(+AGGACA)GATCCGATTATTAGATCTGTGAAAGTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613144701_ymel CAAACTGCTGCCACTCTACAAGCAAATGCCTG(+AGGACA)GATCCCTTCTGATGTGCAGTAACCAGGACTC NGG 10 8 17 8 24 0 100 5 10
    240613144659_lnow AAGGTGAGTCCTGGTTACTGCACATCAGAAGG(+AGGACA)GATCCAGGCATTTGCTTGTAGAGTGGCAGCA NGG 10 8 17 8 24 0 100 5 10